commit f205f16b6f2e5a00a788402c28d5c782c73a0cac Author: Louvenia Bainton Date: Wed Oct 8 00:31:42 2025 +0800 Add How To Implement "Lean Pondering" In A Enterprise diff --git a/How-To-Implement-%22Lean-Pondering%22-In-A-Enterprise.md b/How-To-Implement-%22Lean-Pondering%22-In-A-Enterprise.md new file mode 100644 index 0000000..4c6f8ed --- /dev/null +++ b/How-To-Implement-%22Lean-Pondering%22-In-A-Enterprise.md @@ -0,0 +1 @@ +
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This pore is rimmed with several structurally conserved carbonyl oxygens as well as the hydroxyl group of the invariant tyrosine from the carboxyl terminus of the SET domain (Figure 1 ). These carbonyl and hydroxyl oxygens have been proposed to facilitate the switch of the methyl group throughout catalysis 16 , 21 , 24 As well as, tyrosine residues within the lysine-binding clefts of SET7/9 (Tyr245 and Tyr305) and DIM-5 (Tyr178) hydrogen-bond to the lysine ε-amino group, aligning it for a methyltransfer with AdoMet (Determine 4b,c ). Mutation of Tyr245 or Tyr305 in SET7/9 (Determine 4b ) alters its specificity from an H3 K4 mono-methylase to a tri- and di-methylase, respectively 16 , 21 , whereas an Phe281Tyr mutation in the lysine-binding pocket of DIM-5 (Determine 4c ) converts this protein to an H3 K9 mono- or di-methylase 16 These mutations exemplify the F/Y swap (Determine 1 ) that establishes SET-domain product specificities.

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